Coding

Part:BBa_K4656002

Designed by: Xinji Gu   Group: iGEM23_NMU-China   (2023-08-07)


TPH

TPH also plays a crucial role in the synthesis of serotonin, where the rate-limiting step is catalyzed by TPH to convert tryptophan (an essential amino acid) to 5-hydroxytryptophan (5-HTP).

The synthesis of 5-HT


Usage and Biology

As a neurotransmitter, serotonin plays a role in intestinal motility. If secretion in the gut is insufficient, it can easily lead to the decline of intestinal motility, and then lead to chronic constipation. Therefore, promoting TPH expression is conducive to the treatment of chronic constipation. Experimental results about our 5-HT producing circuit can be found in our composite part BBa K4656007.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 898
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 673
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 240


[edit]
Categories
Parameters
None